/
/
Flotationsbasierte T-Zell-Isolierung, -Aktivierung und -Expansion aus mononukleären Blutproben des menschlichen peripheren Blutes unter Verwendung von Mikrobläschen
Bu içeriği görüntülemek için JoVE aboneliği gereklidir. Oturum açın veya ücretsiz deneme sürümünü başlatın.
Flotationsbasierte T-Zell-Isolierung, -Aktivierung und -Expansion aus mononukleären Blutproben des menschlichen peripheren Blutes unter Verwendung von Mikrobläschen
Bölümler
- 00:04Introduction
- 00:29Isolation of T Cells with Microbubbles Using Positive Selection
- 01:51Co-Stimulation (Activation) of the Positively Selected T Cells
- 02:52Expansion of the Co-Stimulated Cells in Cell Culture Medium
- 03:28Expansion of the T Cells (With or Without Prior Transduction)
- 04:02Harvesting the T Cells and Flow Cytometry
- 05:16Results: Assessment of Viable and Successfully Transduced Cells
- 05:58Conclusion
