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Flotation-Based T Cell Isolation, Activation, and Expansion from Human Peripheral Blood Mononuclear Cell Samples Using Microbubbles
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Flotation-Based T Cell Isolation, Activation, and Expansion from Human Peripheral Blood Mononuclear Cell Samples Using Microbubbles
Bölümler
- 00:04Introduction
- 00:29Isolation of T Cells with Microbubbles Using Positive Selection
- 01:51Co-Stimulation (Activation) of the Positively Selected T Cells
- 02:52Expansion of the Co-Stimulated Cells in Cell Culture Medium
- 03:28Expansion of the T Cells (With or Without Prior Transduction)
- 04:02Harvesting the T Cells and Flow Cytometry
- 05:16Results: Assessment of Viable and Successfully Transduced Cells
- 05:58Conclusion
