Shear processing during hydrogel formation results in the production of microgel suspensions that shear-thin but rapidly restructure following the removal of shear forces. Such materials have been used as a supporting matrix for bioprinting complex, cell-laden structures. Here, methods used to manufacture the supporting bed and compatible bioinks are described.
Senior, J. J., Moakes, R. J. A., Cooke, M. E., Moxon, S. R., Smith, A. M., Grover, L. M. Agarose Fluid Gels Formed by Shear Processing During Gelation for Suspended 3D Bioprinting. J. Vis. Exp. (195), e64458, doi:10.3791/64458 (2023).