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Bioengineering

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JoVE Journal Bioengineering
Agarose Fluid Gels Formed by Shear Processing During Gelation for Suspended 3D Bioprinting
 

Agarose Fluid Gels Formed by Shear Processing During Gelation for Suspended 3D Bioprinting

Article DOI: 10.3791/64458-v • 07:26 min • May 26th, 2023
May 26th, 2023 •
*1, *2, 3, 4, 1, 2
1Department of Pharmacy, University of Huddersfield, 2School of Chemical Engineering, University of Birmingham, 3Chemical and Biological Engineering, University of Colorado Boulder, 4School of Biological Sciences, University of Manchester
* These authors contributed equally

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Shear processing during hydrogel formation results in the production of microgel suspensions that shear-thin but rapidly restructure following the removal of shear forces. Such materials have been used as a supporting matrix for bioprinting complex, cell-laden structures. Here, methods used to manufacture the supporting bed and compatible bioinks are described.

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Agarose Fluid Gels Shear Processing Gelation Suspended 3D Bioprinting Bioinks Conventional Printing Techniques Low Viscosity Bioinks Suspending Medium Regional Variations Chemical Properties Mechanical Properties Native Tissue Dr. Tom Robertson University Of Birmingham Dr. Jessica Senior University Of Huddersfield Rheometer Serrated Geometries Gap Height Viscometry Profiles Shear Rate Controlled Ramp Test
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