Dergi
/
Biyokimya
/
Visualizing the Conformational Dynamics of Membrane Receptors Using Single-Molecule FRET
JoVE Journal
Biyokimya
Bu içeriği görüntülemek için JoVE aboneliği gereklidir.  Oturum açın veya ücretsiz deneme sürümünü başlatın.
JoVE Journal Biyokimya
Visualizing the Conformational Dynamics of Membrane Receptors Using Single-Molecule FRET

Visualizing the Conformational Dynamics of Membrane Receptors Using Single-Molecule FRET

2,933 Views

•

10:59 min

•

August 17, 2022

DOI:

10.3791/64254-v

DOI:
10.3791/64254-v

•

10:59 min
August 17, 2022

•

8 Views
, ,
1Department of Molecular Biosciences,Northwestern University

Özet

Automatically generated

This study presents a detailed procedure to perform single-molecule fluorescence resonance energy transfer (smFRET) experiments on G protein-coupled receptors (GPCRs) using site-specific labeling via unnatural amino acid (UAA) incorporation. The protocol provides a step-by-step guide for smFRET sample preparation, experiments, and data analysis.

Explore More Videos

Single-molecule FRETMembrane ReceptorsConformational DynamicsProtein LabelingMGluR2HEK 293T CellsPEGylationAmino SalinizationDremelAcetonePotassium HydroxideMethanolPoly-L/D-lysineTransfectionAlkyne Cyanine Dyes

Read Article

Cite this Article

Banerjee, C., Liauw, B. W., Vafabakhsh, R. Visualizing the Conformational Dynamics of Membrane Receptors Using Single-Molecule FRET. J. Vis. Exp. (186), e64254, doi:10.3791/64254 (2022).

Download .ris file

Copy

Share Video

.

Copy