ジャーナル
/
生化学
/
Visualizing the Conformational Dynamics of Membrane Receptors Using Single-Molecule FRET
JoVE Journal
生化学
このコンテンツを視聴するには、JoVE 購読が必要です。  サインイン又は無料トライアルを申し込む。
JoVE Journal 生化学
Visualizing the Conformational Dynamics of Membrane Receptors Using Single-Molecule FRET

Visualizing the Conformational Dynamics of Membrane Receptors Using Single-Molecule FRET

2,933 Views

•

10:59 min

•

August 17, 2022

DOI:

10.3791/64254-v

DOI:
10.3791/64254-v

•

10:59 min
August 17, 2022

•

8 Views
, ,
1Department of Molecular Biosciences,Northwestern University

概要

Automatically generated

This study presents a detailed procedure to perform single-molecule fluorescence resonance energy transfer (smFRET) experiments on G protein-coupled receptors (GPCRs) using site-specific labeling via unnatural amino acid (UAA) incorporation. The protocol provides a step-by-step guide for smFRET sample preparation, experiments, and data analysis.

Explore More Videos

Single-molecule FRETMembrane ReceptorsConformational DynamicsProtein LabelingMGluR2HEK 293T CellsPEGylationAmino SalinizationDremelAcetonePotassium HydroxideMethanolPoly-L/D-lysineTransfectionAlkyne Cyanine Dyes

Read Article

Cite this Article

Banerjee, C., Liauw, B. W., Vafabakhsh, R. Visualizing the Conformational Dynamics of Membrane Receptors Using Single-Molecule FRET. J. Vis. Exp. (186), e64254, doi:10.3791/64254 (2022).

Download .ris file

Copy

Share Video

.

Copy