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JoVE Journal Biochemistry
Visualizing the Conformational Dynamics of Membrane Receptors Using Single-Molecule FRET
 

Visualizing the Conformational Dynamics of Membrane Receptors Using Single-Molecule FRET

Article DOI: 10.3791/64254-v • 10:59 min • August 17th, 2022
August 17th, 2022 •
1, 1, 1
1Department of Molecular Biosciences, Northwestern University

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This study presents a detailed procedure to perform single-molecule fluorescence resonance energy transfer (smFRET) experiments on G protein-coupled receptors (GPCRs) using site-specific labeling via unnatural amino acid (UAA) incorporation. The protocol provides a step-by-step guide for smFRET sample preparation, experiments, and data analysis.

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Conformational Dynamics Membrane Receptors Single-molecule FRET Protein Labeling MGluR2 Protein Dynamics Atomic Structure Protein Motion Real-time Visualization Solution Protein-protein Interactions Synthetic Receptors Sample Loading Imaging Optimization Glass Slide Drilling Acetone Sonication Potassium Hydroxide Rinsing Methanol Sonication Amino Salinization
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