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Use of Dual Optical Tweezers and Microfluidics for Single-Molecule Studies
Bölümler
- 00:05Introduction
- 01:16Flow Cell Connections Using a PDMS Flow Cell
- 02:13Attachment of Press Fit Tubing Connectors
- 02:50Attachment of Permanent Assemblies
- 03:39Flow Cell Connections and Preparation
- 04:19Results: The Power Output of the Laser, Fluid Flow Characteristics through a Glass Flow Cell, and Effects on Bead Motion and Opposing Force by Fluid Flow
- 05:59Conclusion
Visual, single-molecule biochemistry studied through microfluidic chambers is greatly facilitated using glass barrel, gas-tight syringes, stable connections of tubing to flow cells, and elimination of bubbles by placing switching valves between the syringes and tubing. The protocol describes dual optical traps that enable visualization of DNA transactions and intermolecular interactions.
