Se requiere una suscripción a JoVE para ver este contenido. Inicie sesión o comience su prueba gratuita.
Use of Dual Optical Tweezers and Microfluidics for Single-Molecule Studies
1,932 Views
•
06:53 min
•
November 18, 2022
DOI:
Summary
Automatically generated
Visual, single-molecule biochemistry studied through microfluidic chambers is greatly facilitated using glass barrel, gas-tight syringes, stable connections of tubing to flow cells, and elimination of bubbles by placing switching valves between the syringes and tubing. The protocol describes dual optical traps that enable visualization of DNA transactions and intermolecular interactions.
Read Article
Cite this Article
Bianco, P. R. Use of Dual Optical Tweezers and Microfluidics for Single-Molecule Studies. J. Vis. Exp. (189), e64023, doi:10.3791/64023 (2022).
Copy