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Rapid Antibody Glycoengineering in Chinese Hamster Ovary Cells
1Department of Chemical Engineering,Imperial College London, 2Imperial College Centre for Synthetic Biology,Imperial College London, 3BioPharm Process Research,GlaxoSmithKline Research and Development, 4Department of Life Science,Imperial College London, 5School of Chemical & Bioprocess Engineering,University College Dublin
Bölümler
- 00:04Introduction
- 01:00DsiRNA Transfection
- 02:17Buffer Exchange and Sample Concentration
- 03:19Glycan Analysis
- 05:18Results: Confirmation of RNA Interference and Effect of Fut8 Knockdown
- 06:12Conclusion
The glycosylation pattern of an antibody determines its clinical performance, thus industrial and academic efforts to control glycosylation persist. Since typical glycoengineering campaigns are time- and labor-intensive, the generation of a rapid protocol to characterize the impact of glycosylation genes using transient silencing would prove useful.
