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Journal / Bioengineering / Rapid Antibody Glycoengineering in Chinese…
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Bioengineering

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JoVE Journal Bioengineering
Rapid Antibody Glycoengineering in Chinese Hamster Ovary Cells
 

Rapid Antibody Glycoengineering in Chinese Hamster Ovary Cells

Article DOI: 10.3791/63872-v • 06:53 min • June 2nd, 2022
June 2nd, 2022 •
*1,2, *1,3, 1,4, 5, 5, 4, 1,2, 1
1Department of Chemical Engineering, Imperial College London, 2Imperial College Centre for Synthetic Biology, Imperial College London, 3BioPharm Process Research, GlaxoSmithKline Research and Development, 4Department of Life Science, Imperial College London, 5School of Chemical & Bioprocess Engineering, University College Dublin
* These authors contributed equally

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The glycosylation pattern of an antibody determines its clinical performance, thus industrial and academic efforts to control glycosylation persist. Since typical glycoengineering campaigns are time- and labor-intensive, the generation of a rapid protocol to characterize the impact of glycosylation genes using transient silencing would prove useful.

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Rapid Antibody Glycoengineering Chinese Hamster Ovary Cells Glycosylation Enzymes Glyco-profile Enzyme Functionalities High-throughput Screening Altered Glycosylation Profiles Antibody Activities Biopharmaceutical Companies Critical Quality Attribute Aberrant Glycosylation Pharmacological Research Biomedical Research Mammalian Cell Transfection Western Blots Chinese Hamster Ovary Cells Density And Viability Biosafety Cabinet Cleaning RNase Inhibitor Solution Cell Pellet Pre-warmed Medium DsiRNA Master Mix Electroporation Cuvette
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