Dergi
/
Nörobilim
/
Demonstrating Hairy and Glabrous Skin Innervation in a 3D Pattern Using Multiple Fluorescent Staining and Tissue Clearing Approaches
JoVE Journal
Nörobilim
Bu içeriği görüntülemek için JoVE aboneliği gereklidir.  Oturum açın veya ücretsiz deneme sürümünü başlatın.
JoVE Journal Nörobilim
Demonstrating Hairy and Glabrous Skin Innervation in a 3D Pattern Using Multiple Fluorescent Staining and Tissue Clearing Approaches

Demonstrating Hairy and Glabrous Skin Innervation in a 3D Pattern Using Multiple Fluorescent Staining and Tissue Clearing Approaches

DOI:
10.3791/63807-v

•

05:23 min

•

May 20, 2022

•

, , , , , , , , , ,
1Institute of Acupuncture and Moxibustion,China Academy of Chinese Medical Sciences

Bölümler

  • 00:05Introduction
  • 00:43Perfusion, Triple Fluorescent Staining, and Tissue Clearing Treatment
  • 03:10Results: Enhanced Understanding of the Skin Innervation via Immunofluorescence and a 3D View of the Cutaneous Nerve Fibers in the Hairy and Glabrous Skin
  • 04:31Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

The thickness of tissue sections limited the morphological study of the skin innervation. The present protocol describes a unique tissue clearing technique to visualize cutaneous nerve fibers in thick 300 µm tissue sections under confocal microscopy.

Etiketler

Fluorescent StainingSkin InnervationCGRPLYVE-13D ImagingConfocal Microscopy

Article

Embed

ÇALMA LİSTESİNE EKLE

Usage Statistics

İlgili Videolar

Cross-Modal Multivariate Pattern Analysis

Tissue Preparation and Immunostaining of Mouse Sensory Nerve Fibers Innervating Skin and Limb Bones

3D Modeling of the Lateral Ventricles and Histological Characterization of Periventricular Tissue in Humans and Mouse

Analysis of Developing Tooth Germ Innervation Using Microfluidic Co-culture Devices

In Utero Electroporation Approaches to Study the Excitability of Neuronal Subpopulations and Single-cell Connectivity

Quantification of Endosome and Lysosome Motilities in Cultured Neurons Using Fluorescent Probes

Novel Passive Clearing Methods for the Rapid Production of Optical Transparency in Whole CNS Tissue

A Rat Model of Central Fatigue Using a Modified Multiple Platform Method

A Hydrophobic Tissue Clearing Method for Rat Brain Tissue

In vivo Imaging of Fully Active Brain Tissue in Awake Zebrafish Larvae and Juveniles by Skull and Skin Removal

Read Article