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Demonstrating Hairy and Glabrous Skin Innervation in a 3D Pattern Using Multiple Fluorescent Staining and Tissue Clearing Approaches
 

Demonstrating Hairy and Glabrous Skin Innervation in a 3D Pattern Using Multiple Fluorescent Staining and Tissue Clearing Approaches

Article DOI: 10.3791/63807-v • 05:23 min • May 20th, 2022
May 20th, 2022 •
*1, *1, *1, 1, 1, 1, 1, 1, 1, 1, 1
1Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences
* These authors contributed equally

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The thickness of tissue sections limited the morphological study of the skin innervation. The present protocol describes a unique tissue clearing technique to visualize cutaneous nerve fibers in thick 300 µm tissue sections under confocal microscopy.

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Hairy Skin Innervation Glabrous Skin Innervation 3D Pattern Fluorescent Staining Tissue Clearing Confocal Microscope Nerve Fibers Tissue Cleaning Protocol Perfusion Frozen Section Aldehyde Fixation Agarose Embedding Vibratory Microtome Thickness PBS Incubation Triton X-100 Solution Blocking Buffer Primary Antibodies
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