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Demonstrating Hairy and Glabrous Skin Innervation in a 3D Pattern Using Multiple Fluorescent Staining and Tissue Clearing Approaches
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Demonstrating Hairy and Glabrous Skin Innervation in a 3D Pattern Using Multiple Fluorescent Staining and Tissue Clearing Approaches
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May 20, 2022
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The thickness of tissue sections limited the morphological study of the skin innervation. The present protocol describes a unique tissue clearing technique to visualize cutaneous nerve fibers in thick 300 µm tissue sections under confocal microscopy.
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Wang, X., Cao, W., Shi, J., Zhang, X., Qu, Z., Xu, D., Wan, H., Su, Y., He, W., Jing, X., Bai, W. Demonstrating Hairy and Glabrous Skin Innervation in a 3D Pattern Using Multiple Fluorescent Staining and Tissue Clearing Approaches. J. Vis. Exp. (183), e63807, doi:10.3791/63807 (2022).
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