A high-speed and open-top ultraviolet photoacoustic microscope that can provide histological images intraoperatively for surgical margin analysis is demonstrated, including the system configuration, optical alignment, sample preparation, and experimental procedures.
Li, X., Kang, L., Lo, C. T. K., Tsang, V. T. C., Wong, T. T. W. High-Speed Ultraviolet Photoacoustic Microscopy for Histological Imaging with Virtual-Staining assisted by Deep Learning. J. Vis. Exp. (182), e63649, doi:10.3791/63649 (2022).