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High-Speed Ultraviolet Photoacoustic Microscopy for Histological Imaging with Virtual-Staining assisted by Deep Learning
JoVE Revista
Bioingeniería
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JoVE Revista Bioingeniería
High-Speed Ultraviolet Photoacoustic Microscopy for Histological Imaging with Virtual-Staining assisted by Deep Learning

High-Speed Ultraviolet Photoacoustic Microscopy for Histological Imaging with Virtual-Staining assisted by Deep Learning

2,560 Views

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09:31 min

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April 28, 2022

DOI:

10.3791/63649-v

DOI:
10.3791/63649-v

•

09:31 min
April 28, 2022

•

12 Views
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1Translational and Advanced Bioimaging Laboratory, Department of Chemical and Biological Engineering,Hong Kong University of Science and Technology

Summary

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A high-speed and open-top ultraviolet photoacoustic microscope that can provide histological images intraoperatively for surgical margin analysis is demonstrated, including the system configuration, optical alignment, sample preparation, and experimental procedures.

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Ultraviolet Photoacoustic MicroscopyHistological ImagingVirtual StainingDeep LearningIntraoperative Surgical MonitoringTumor ResectionPositive Surgical MarginQ-switch Diode-pumped Solid-state LaserGalvanometer MirrorUltrasound TransducerData AcquisitionSample HolderAlignment

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Cite this Article

Li, X., Kang, L., Lo, C. T. K., Tsang, V. T. C., Wong, T. T. W. High-Speed Ultraviolet Photoacoustic Microscopy for Histological Imaging with Virtual-Staining assisted by Deep Learning. J. Vis. Exp. (182), e63649, doi:10.3791/63649 (2022).

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