Journal
/
Biotechnik
/
High-Speed Ultraviolet Photoacoustic Microscopy for Histological Imaging with Virtual-Staining assisted by Deep Learning
JoVE Journal
Biotechnik
This content is Free Access.
JoVE Journal Biotechnik
High-Speed Ultraviolet Photoacoustic Microscopy for Histological Imaging with Virtual-Staining assisted by Deep Learning

High-Speed Ultraviolet Photoacoustic Microscopy for Histological Imaging with Virtual-Staining assisted by Deep Learning

2,560 Views

•

09:31 min

•

April 28, 2022

DOI:

10.3791/63649-v

DOI:
10.3791/63649-v

•

09:31 min
April 28, 2022

•

12 Views
, , , ,
1Translational and Advanced Bioimaging Laboratory, Department of Chemical and Biological Engineering,Hong Kong University of Science and Technology

Summary

Automatically generated

A high-speed and open-top ultraviolet photoacoustic microscope that can provide histological images intraoperatively for surgical margin analysis is demonstrated, including the system configuration, optical alignment, sample preparation, and experimental procedures.

Explore More Videos

Ultraviolet Photoacoustic MicroscopyHistological ImagingVirtual StainingDeep LearningIntraoperative Surgical MonitoringTumor ResectionPositive Surgical MarginQ-switch Diode-pumped Solid-state LaserGalvanometer MirrorUltrasound TransducerData AcquisitionSample HolderAlignment

Read Article

Cite this Article

Li, X., Kang, L., Lo, C. T. K., Tsang, V. T. C., Wong, T. T. W. High-Speed Ultraviolet Photoacoustic Microscopy for Histological Imaging with Virtual-Staining assisted by Deep Learning. J. Vis. Exp. (182), e63649, doi:10.3791/63649 (2022).

Download .ris file

Copy

Share Video

.

Copy