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Analyses of Actin Dynamics, Clutch Coupling and Traction Force for Growth Cone Advance
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Analyses of Actin Dynamics, Clutch Coupling and Traction Force for Growth Cone Advance

Analyses of Actin Dynamics, Clutch Coupling and Traction Force for Growth Cone Advance

2,995 Views

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07:53 min

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October 21, 2021

DOI:

10.3791/63227-v

DOI:
10.3791/63227-v

•

07:53 min
October 21, 2021

•

7 Views
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1Laboratory of Systems Neurobiology and Medicine, Division of Biological Science,Nara Institute of Science and Technology, 2Laboratory of Data-Driven Biology, Division of Biological Science,Nara Institute of Science and Technology

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To advance, growth cones must exert traction forces against the external environment. The generation of traction forces is dependent on actin dynamics and clutch coupling. The present study describes methods for analyzing actin dynamics, clutch coupling and traction forces for growth cone advance.

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Minegishi, T., Fujikawa, R., Kastian, R. F., Sakumura, Y., Inagaki, N. Analyses of Actin Dynamics, Clutch Coupling and Traction Force for Growth Cone Advance. J. Vis. Exp. (176), e63227, doi:10.3791/63227 (2021).

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