To advance, growth cones must exert traction forces against the external environment. The generation of traction forces is dependent on actin dynamics and clutch coupling. The present study describes methods for analyzing actin dynamics, clutch coupling and traction forces for growth cone advance.
Minegishi, T., Fujikawa, R., Kastian, R. F., Sakumura, Y., Inagaki, N. Analyses of Actin Dynamics, Clutch Coupling and Traction Force for Growth Cone Advance. J. Vis. Exp. (176), e63227, doi:10.3791/63227 (2021).