<em>Caenorhabditis elegans</em> as a Model System for Discovering Bioactive Compounds Against Polyglutamine-Mediated Neurotoxicity

Qiangqiang Wang; Ju Zhang; Yiyi Jiang; Yue Xiao; Xiaomin Li; Xinliang Mao; Zebo Huang

doi:10.3791/63081

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Caenorhabditis elegans as a Model System for Discovering Bioactive Compounds Against Polyglutamine-Mediated Neurotoxicity

JoVE Journal
Nörobilim

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JoVE Journal Nörobilim

Caenorhabditis elegans as a Model System for Discovering Bioactive Compounds Against Polyglutamine-Mediated Neurotoxicity

Caenorhabditis elegans as a Model System for Discovering Bioactive Compounds Against Polyglutamine-Mediated Neurotoxicity

DOI:

10.3791/63081-v

•

08:16 min

•

September 21, 2021

•

Qiangqiang Wang*¹, Ju Zhang*², Yiyi Jiang³, Yue Xiao, Xiaomin Li, Xinliang Mao, Zebo Huang

¹Institute for Food Nutrition and Human Health, School of Food Science and Engineering,South China University of Technology, ²School of Bioscience and Bioengineering,Hebei University of Economics and Business, ³Perfect Life & Health Institute

Bölümler

00:05Introduction
01:12PolyQ Aggregation Assay
03:29PolyQ-Mediated Neurotoxicity Assays
06:18Results: Analysis of the Overall Neuroprotective Capacity of Astragalan and its Effect on PolyQ40 Aggregation, Neuronal Death, and Behavioral Dysfunction
07:51Conclusion

Özet

Otomatik Çeviri

Here, we present a protocol to assess the neuroprotective activities of test compounds in Caenorhabditis elegans, including polyglutamine aggregation, neuronal death, and chemoavoidance behavior, as well as an exemplary integration of multiple phenotypes.

Etiketler

C. Elegans Polyglutamine Neurotoxicity Huntington’s Disease Animal Model High-throughput Screening Polyglutamine Aggregates Fluorescence Imaging Image Analysis

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