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Caenorhabditis elegans as a Model System for Discovering Bioactive Compounds Against Polyglutamine-Mediated Neurotoxicity
JoVE Revista
Neurociencias
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JoVE Revista Neurociencias
Caenorhabditis elegans as a Model System for Discovering Bioactive Compounds Against Polyglutamine-Mediated Neurotoxicity

Caenorhabditis elegans as a Model System for Discovering Bioactive Compounds Against Polyglutamine-Mediated Neurotoxicity

DOI:
10.3791/63081-v

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08:16 min

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September 21, 2021

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, , , , , ,
1Institute for Food Nutrition and Human Health, School of Food Science and Engineering,South China University of Technology, 2School of Bioscience and Bioengineering,Hebei University of Economics and Business, 3Perfect Life & Health Institute

Capítulos

  • 00:05Introduction
  • 01:12PolyQ Aggregation Assay
  • 03:29PolyQ-Mediated Neurotoxicity Assays
  • 06:18Results: Analysis of the Overall Neuroprotective Capacity of Astragalan and its Effect on PolyQ40 Aggregation, Neuronal Death, and Behavioral Dysfunction
  • 07:51Conclusion

Summary

Traducción Automática

Traducción Automática

Here, we present a protocol to assess the neuroprotective activities of test compounds in Caenorhabditis elegans, including polyglutamine aggregation, neuronal death, and chemoavoidance behavior, as well as an exemplary integration of multiple phenotypes.

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Keywords: C. ElegansPolyglutamineNeurotoxicityHuntington’s DiseaseAnimal ModelHigh-throughput ScreeningPolyglutamine AggregatesFluorescence ImagingImage Analysis

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