Journal
/
Neurosciences
/
Caenorhabditis elegans as a Model System for Discovering Bioactive Compounds Against Polyglutamine-Mediated Neurotoxicity
Journal JoVE
Neurosciences
This content is Free Access.
Journal JoVE Neurosciences
Caenorhabditis elegans as a Model System for Discovering Bioactive Compounds Against Polyglutamine-Mediated Neurotoxicity

Caenorhabditis elegans as a Model System for Discovering Bioactive Compounds Against Polyglutamine-Mediated Neurotoxicity

DOI:
10.3791/63081-v

•

08:16 min

•

September 21, 2021

•

, , , , , ,
1Institute for Food Nutrition and Human Health, School of Food Science and Engineering,South China University of Technology, 2School of Bioscience and Bioengineering,Hebei University of Economics and Business, 3Perfect Life & Health Institute

Chapitres

  • 00:05Introduction
  • 01:12PolyQ Aggregation Assay
  • 03:29PolyQ-Mediated Neurotoxicity Assays
  • 06:18Results: Analysis of the Overall Neuroprotective Capacity of Astragalan and its Effect on PolyQ40 Aggregation, Neuronal Death, and Behavioral Dysfunction
  • 07:51Conclusion

Summary

Traduction automatique

Traduction automatique

Here, we present a protocol to assess the neuroprotective activities of test compounds in Caenorhabditis elegans, including polyglutamine aggregation, neuronal death, and chemoavoidance behavior, as well as an exemplary integration of multiple phenotypes.

Tags

Keywords: C. ElegansPolyglutamineNeurotoxicityHuntington’s DiseaseAnimal ModelHigh-throughput ScreeningPolyglutamine AggregatesFluorescence ImagingImage Analysis

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/63081?article_type=v

Assaying β-amyloid Toxicity using a Transgenic C. elegans Model
check_url/fr/63081?article_type=v

Paired Nanoinjection and Electrophysiology Assay to Screen for Bioactivity of Compounds using the Drosophila melanogaster Giant Fiber System
check_url/fr/63081?article_type=v

A Caenorhabditis elegans Model System for Amylopathy Study
check_url/fr/63081?article_type=v

Methods for Studying the Mechanisms of Action of Antipsychotic Drugs in Caenorhabditis elegans
check_url/fr/63081?article_type=v

Real-Time Impedance-based Cell Analyzer as a Tool to Delineate Molecular Pathways Involved in Neurotoxicity and Neuroprotection in a Neuronal Cell Line
check_url/fr/63081?article_type=v

The Olfactory System as a Model to Study Axonal Growth Patterns and Morphology In Vivo
check_url/fr/63081?article_type=v

An Assay for Measuring the Effects of Ethanol on the Locomotion Speed of Caenorhabditis elegans
check_url/fr/63081?article_type=v

Agarose Microchambers for Long-term Calcium Imaging of Caenorhabditis elegans
check_url/fr/63081?article_type=v

Microglia as a Surrogate Biosensor to Determine Nanoparticle Neurotoxicity
check_url/fr/63081?article_type=v

A Caenorhabditis elegans Nutritional-status Based Copper Aversion Assay

Read Article