Dergi
/
Nörobilim
/
A Scalable Model to Study the Effects of Blunt-Force Injury in Adult Zebrafish
JoVE Journal
Nörobilim
Bu içeriği görüntülemek için JoVE aboneliği gereklidir.  Oturum açın veya ücretsiz deneme sürümünü başlatın.
JoVE Journal Nörobilim
A Scalable Model to Study the Effects of Blunt-Force Injury in Adult Zebrafish

A Scalable Model to Study the Effects of Blunt-Force Injury in Adult Zebrafish

DOI:
10.3791/62709-v

•

08:13 min

•

May 31, 2021

•

, , ,
1Department of Biological Sciences,Notre Dame Üniversitesi, 2Center for Zebrafish Research,Notre Dame Üniversitesi, 3Center for Stem Cells and Regenerative Medicine,Notre Dame Üniversitesi

Bölümler

  • 00:04Introduction
  • 00:38Traumatic Brain Injury Paradigm
  • 02:26Brain Dissection
  • 03:54Edema Studies in the Zebrafish Brain
  • 04:42Labeling Cellular Proliferation Across the Neuroaxis and Preparing Fixed Tissue
  • 05:33Results: Effects of Blunt-Force Injury in Adult Zebrafish
  • 07:28Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

We modified the Marmarou weight drop model for adult zebrafish to examine a breadth of pathologies following blunt-force traumatic brain injury (TBI) and the mechanisms underlying subsequent neuronal regeneration. This blunt-force TBI model is scalable, induces a mild, moderate, or severe TBI, and recapitulates injury heterogeneity observed in human TBI.

Etiketler

ZebrafishBlunt-force InjuryTraumatic Brain InjuryTBIModeling ClaySteel DiscBall BearingInjury SeverityDissectionOptic NerveParietal Plate

Article

Embed

ÇALMA LİSTESİNE EKLE

Usage Statistics

İlgili Videolar

An Optic Nerve Crush Injury Murine Model to Study Retinal Ganglion Cell Survival

Micromanipulation of Gene Expression in the Adult Zebrafish Brain Using Cerebroventricular Microinjection of Morpholino Oligonucleotides

Electrophysiological Recording in the Brain of Intact Adult Zebrafish

Stab Wound Injury of the Zebrafish Adult Telencephalon: A Method to Investigate Vertebrate Brain Neurogenesis and Regeneration

The Olfactory System as a Model to Study Axonal Growth Patterns and Morphology In Vivo

A Simple Neuronal Mechanical Injury Methodology to Study Drosophila Motor Neuron Degeneration

Effects of Blast-induced Neurotrauma on Pressurized Rodent Middle Cerebral Arteries

Facial Nerve Surgery in the Rat Model to Study Axonal Inhibition and Regeneration

Using Zebrafish Larvae to Study the Pathological Consequences of Hemorrhagic Stroke

Combining Behavior and EEG to Study the Effects of Mindfulness Meditation on Episodic Memory

Read Article