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A Scalable Model to Study the Effects of Blunt-Force Injury in Adult Zebrafish
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Neuroscienze
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JoVE Journal Neuroscienze
A Scalable Model to Study the Effects of Blunt-Force Injury in Adult Zebrafish

A Scalable Model to Study the Effects of Blunt-Force Injury in Adult Zebrafish

DOI:
10.3791/62709-v

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08:13 min

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May 31, 2021

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, , ,
1Department of Biological Sciences,University of Notre Dame, 2Center for Zebrafish Research,University of Notre Dame, 3Center for Stem Cells and Regenerative Medicine,University of Notre Dame

Capitoli

  • 00:04Introduction
  • 00:38Traumatic Brain Injury Paradigm
  • 02:26Brain Dissection
  • 03:54Edema Studies in the Zebrafish Brain
  • 04:42Labeling Cellular Proliferation Across the Neuroaxis and Preparing Fixed Tissue
  • 05:33Results: Effects of Blunt-Force Injury in Adult Zebrafish
  • 07:28Conclusion

Summary

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We modified the Marmarou weight drop model for adult zebrafish to examine a breadth of pathologies following blunt-force traumatic brain injury (TBI) and the mechanisms underlying subsequent neuronal regeneration. This blunt-force TBI model is scalable, induces a mild, moderate, or severe TBI, and recapitulates injury heterogeneity observed in human TBI.

Tags

ZebrafishBlunt-force InjuryTraumatic Brain InjuryTBIModeling ClaySteel DiscBall BearingInjury SeverityDissectionOptic NerveParietal Plate

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