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Transpupillary Two-Photon In Vivo Imaging of the Mouse Retina
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February 13, 2021
DOI:
1John F. Hardesty, MD Department of Ophthalmology and Visual Sciences,Washington Üniversitesi Tıp Fakültesi, 2Graduate Program in Neuroscience,Washington Üniversitesi Tıp Fakültesi, 3Nörobilim Bölümü,Washington Üniversitesi Tıp Fakültesi, 4Hope Center for Neurological Disorders,Washington Üniversitesi Tıp Fakültesi
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In vivo imaging is a powerful tool for the study of biology in health and disease. This protocol describes transpupillary imaging of the mouse retina with a standard two-photon microscope. It also demonstrates different in vivoimaging methods to fluorescently label multiple cellular cohorts of the retina.
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Wang, Z., McCracken, S., Williams, P. R. Transpupillary Two-Photon In Vivo Imaging of the Mouse Retina. J. Vis. Exp. (168), e61970, doi:10.3791/61970 (2021).
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