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Transpupillary Two-Photon In Vivo Imaging of the Mouse Retina
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Transpupillary Two-Photon In Vivo Imaging of the Mouse Retina

Transpupillary Two-Photon In Vivo Imaging of the Mouse Retina

3,573 Views

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09:03 min

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February 13, 2021

DOI:

10.3791/61970-v

DOI:
10.3791/61970-v

•

09:03 min
February 13, 2021

•

33 Views
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1John F. Hardesty, MD Department of Ophthalmology and Visual Sciences,Washington University 医学部, 2Graduate Program in Neuroscience,Washington University 医学部, 3神経科学科,Washington University 医学部, 4Hope Center for Neurological Disorders,Washington University 医学部

概要

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In vivo imaging is a powerful tool for the study of biology in health and disease. This protocol describes transpupillary imaging of the mouse retina with a standard two-photon microscope. It also demonstrates different in vivoimaging methods to fluorescently label multiple cellular cohorts of the retina.

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Cite this Article

Wang, Z., McCracken, S., Williams, P. R. Transpupillary Two-Photon In Vivo Imaging of the Mouse Retina. J. Vis. Exp. (168), e61970, doi:10.3791/61970 (2021).

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