This protocol aims to transplant a 3D bioprinted patch onto the epicardium of infarcted mice modeling heart failure. It includes details regarding anesthesia, the surgical chest opening, permanent ligation of the left anterior descending (LAD) coronary artery and application of a bioprinted patch onto the infarcted area of the heart.
Roche, C. D., Gentile, C. Transplantation of a 3D Bioprinted Patch in a Murine Model of Myocardial Infarction. J. Vis. Exp. (163), e61675, doi:10.3791/61675 (2020).