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Transplantation of a 3D Bioprinted Patch in a Murine Model of Myocardial Infarction
JoVE 杂志
生物工程
Author Produced
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JoVE 杂志 生物工程
Transplantation of a 3D Bioprinted Patch in a Murine Model of Myocardial Infarction

Transplantation of a 3D Bioprinted Patch in a Murine Model of Myocardial Infarction

DOI:
10.3791/61675-v

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07:47 min

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September 26, 2020

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,
1The University of Sydney, 2University of Technology Sydney (UTS), 3The Royal North Shore Hospital, 4University Hospital of Wales

Chapters

  • 00:00Introduction
  • 00:42Surgical Procedure
  • 01:18Preparing the Surgical Field
  • 01:31Left Lateral Thoracotomy
  • 02:00Left Anterior Descending (LAD) Permanent Coronary Artery Ligation
  • 02:583D Bioprinting and Culturing Patches (7-14 days before transplantation)
  • 04:19Transplantation of the Bioprinted Patch onto the Epicardium
  • 05:39Mouse Recovery
  • 06:29Representative Results
  • 06:45Conclusion

Summary

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This protocol aims to transplant a 3D bioprinted patch onto the epicardium of infarcted mice modeling heart failure. It includes details regarding anesthesia, the surgical chest opening, permanent ligation of the left anterior descending (LAD) coronary artery and application of a bioprinted patch onto the infarcted area of the heart.

Tags

Keywords: 3D BioprintingStem CellHeart PatchMyocardial InfarctionCardiac CellsHydrogelLAD LigationMouse ModelTransplantation

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