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髄膜炎菌 人工多能性幹細胞由来脳内皮細胞の感染
JoVE Journal
İmmünoloji ve Enfeksiyon
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JoVE Journal İmmünoloji ve Enfeksiyon
Neisseria meningitidis Infection of Induced Pluripotent Stem-Cell Derived Brain Endothelial Cells

髄膜炎菌 人工多能性幹細胞由来脳内皮細胞の感染

DOI:

10:12 min

July 14, 2020

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Bölümler

  • 00:05Introduction
  • 00:42IMR90-4 Cell Culture Maintenance
  • 02:15Differentiation of Brain Endothelial Cells from Human iPSCs
  • 05:57Transendothelial Electrical Resistance (TEER) as a Measure of Barrier Tightness
  • 06:35Preparation of Bacteria and Infection of iPSC-BECs
  • 08:22Results: Upregulation of Cytokines by iPSC-BECs upon Infection with Neisseria meningitidis
  • 09:16Conclusion

Özet

Otomatik Çeviri

ここに記述されているプロトコルは伝染のための Neisseriaの髄膜炎 の準備、および他の分子分析のためのサンプル収集を分化させる誘導された多能性幹細胞によって得られる脳そっくりのendothelialセル、準備の主要なステップを強調する。

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