<em>Neisseria meningitidis</em> Infection of Induced Pluripotent Stem-Cell Derived Brain Endothelial Cells

Leo  M. Endres; Sarah F. Hathcock; Alexandra Schubert-Unkmeir; Brandon J. Kim

doi:10.3791/61400

Journal

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Immunology and Infection

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Neisseria meningitidis Infection of Induced Pluripotent Stem-Cell Derived Brain Endothelial Cells

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Immunology and Infection

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JoVE Journal Immunology and Infection

Neisseria meningitidis Infection of Induced Pluripotent Stem-Cell Derived Brain Endothelial Cells

Neisseria meningitidis Infection of Induced Pluripotent Stem-Cell Derived Brain Endothelial Cells

DOI:

10.3791/61400-v

•

10:12 min

•

July 14, 2020

•

Leo M. Endres, Sarah F. Hathcock, Alexandra Schubert-Unkmeir, Brandon J. Kim²

¹Institute for Hygiene and Microbiology,University of Würzburg, ²Department of Biological Sciences,University of Alabama

Chapters

00:05Introduction
00:42IMR90-4 Cell Culture Maintenance
02:15Differentiation of Brain Endothelial Cells from Human iPSCs
05:57Transendothelial Electrical Resistance (TEER) as a Measure of Barrier Tightness
06:35Preparation of Bacteria and Infection of iPSC-BECs
08:22Results: Upregulation of Cytokines by iPSC-BECs upon Infection with Neisseria meningitidis
09:16Conclusion

Summary

Automatic Translation

The protocol described here highlights the major steps in the differentiating induced pluripotent stem-cell derived brain-like endothelial cells, the preparation of Neisseria meningitidis for infection, and sample collection for other molecular analyses.

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