The protocol described here highlights the major steps in the differentiating induced pluripotent stem-cell derived brain-like endothelial cells, the preparation of Neisseria meningitidis for infection, and sample collection for other molecular analyses.
Endres, L. M., Hathcock, S. F., Schubert-Unkmeir, A., Kim, B. J. Neisseria meningitidis Infection of Induced Pluripotent Stem-Cell Derived Brain Endothelial Cells. J. Vis. Exp. (161), e61400, doi:10.3791/61400 (2020).