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Studying Effects of Cigarette Smoke on Pseudomonas Infection in Lung Epithelial Cells
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JoVE Journal İmmünoloji ve Enfeksiyon
Studying Effects of Cigarette Smoke on Pseudomonas Infection in Lung Epithelial Cells

Studying Effects of Cigarette Smoke on Pseudomonas Infection in Lung Epithelial Cells

5,700 Views

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09:15 min

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May 11, 2020

DOI:

10.3791/61163-v

DOI:
10.3791/61163-v

•

09:15 min
May 11, 2020

•

2 Views
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1Acute Lung Injury Center of Excellence, Pulmonary, Allergy, Critical Care Medicine, Department of Medicine,University of Pittsburgh School of Medicine

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Described here is a protocol to study how cigarette smoke extract affects bacterial colonization in lung epithelial cells.

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Cigarette SmokePseudomonasLung Epithelial CellsBacterial LoadCell CultureSmoke ExtractionBacterial InfectionCell CountingCell SuspensionCell Preparation

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Li, T., Long, C., Fanning, K. V., Zou, C. Studying Effects of Cigarette Smoke on Pseudomonas Infection in Lung Epithelial Cells. J. Vis. Exp. (159), e61163, doi:10.3791/61163 (2020).

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