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使用主机范围和视觉选择快速、无缝地生成重组痘病毒
JoVE Journal
İmmünoloji ve Enfeksiyon
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JoVE Journal İmmünoloji ve Enfeksiyon
Rapid, Seamless Generation of Recombinant Poxviruses using Host Range and Visual Selection
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使用主机范围和视觉选择快速、无缝地生成重组痘病毒

DOI:
10.3791/61049-v

•

09:25 min

•

May 24, 2020

•

, , , ,
1Department of Medial Microbiology and Immunology,School of Medicine, University of California Davis, 2Department of Microbiology and Immunology,University of Texas Medical Branch at Galveston

Bölümler

  • 00:04Introduction
  • 01:03Recombination Vector Generation
  • 03:47Recombinant Virus Generation
  • 06:31Results: Representative Recombinant Poxvirus Generation
  • 08:36Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

这是一种使用宿主范围选择和重组病毒的可视化识别来生成"无疤痕"重组病毒的方法。

Etiketler

Recombinant PoxvirusesHost Range SelectionFluorescent MarkersHomologous RecombinationTransient Dominant SelectionVaccinia VirusForeign Gene ExpressionVaccinesPCRDNA Gel ExtractionDNA AssemblyE. Coli Transformation

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