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Rapid, Seamless Generation of Recombinant Poxviruses using Host Range and Visual Selection
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Rapid, Seamless Generation of Recombinant Poxviruses using Host Range and Visual Selection

Rapid, Seamless Generation of Recombinant Poxviruses using Host Range and Visual Selection

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09:25 min

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May 24, 2020

DOI:

10.3791/61049-v

DOI:
10.3791/61049-v

•

09:25 min
May 24, 2020

•

1 Views
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1Department of Medial Microbiology and Immunology,School of Medicine, University of California Davis, 2Department of Microbiology and Immunology,University of Texas Medical Branch at Galveston

Summary

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This is a method to generate “scarless” recombinant vaccinia viruses using host-range selection and visual identification of recombinant viruses.

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Vipat, S., Brennan, G., Park, C., Haller, S. L., Rothenburg, S. Rapid, Seamless Generation of Recombinant Poxviruses using Host Range and Visual Selection. J. Vis. Exp. (159), e61049, doi:10.3791/61049 (2020).

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