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Rapid, Seamless Generation of Recombinant Poxviruses using Host Range and Visual Selection
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Rapid, Seamless Generation of Recombinant Poxviruses using Host Range and Visual Selection

Rapid, Seamless Generation of Recombinant Poxviruses using Host Range and Visual Selection

5,142 Views

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09:25 min

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May 24, 2020

DOI:

10.3791/61049-v

DOI:
10.3791/61049-v

•

09:25 min
May 24, 2020

•

1 Views
, , , ,
1Department of Medial Microbiology and Immunology,School of Medicine, University of California Davis, 2Department of Microbiology and Immunology,University of Texas Medical Branch at Galveston

Summary

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This is a method to generate “scarless” recombinant vaccinia viruses using host-range selection and visual identification of recombinant viruses.

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Cite this Article

Vipat, S., Brennan, G., Park, C., Haller, S. L., Rothenburg, S. Rapid, Seamless Generation of Recombinant Poxviruses using Host Range and Visual Selection. J. Vis. Exp. (159), e61049, doi:10.3791/61049 (2020).

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