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用于活、完整组织中细胞分部分析的果蝇拉瓦尔和普帕尔睾试验的制备
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Gelişim Biyolojisi
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JoVE Journal Gelişim Biyolojisi
Preparation of Drosophila Larval and Pupal Testes for Analysis of Cell Division in Live, Intact Tissue
Please note that all translations are automatically generated. Click here for the English version.

用于活、完整组织中细胞分部分析的果蝇拉瓦尔和普帕尔睾试验的制备

7,710 Views

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08:05 min

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May 19, 2020

DOI:

10.3791/60961-v

DOI:
10.3791/60961-v

•

08:05 min
May 19, 2020

•

8 Views
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1Department of Anatomy, Physiology, and Genetics, F. Edward Hébert School of Medicine,Uniformed Services University of the Health Sciences

Özet

Automatically generated

该协议的目的是使用果蝇美细胞精细胞，通过活细胞和固定细胞显微镜分析完整组织中细胞分裂。该协议演示了如何从果蝇幼虫和早期幼虫中分离出完整、完整的睾口，以及如何处理和安装它们进行显微镜检查。

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Karabasheva, D., Smyth, J. T. Preparation of Drosophila Larval and Pupal Testes for Analysis of Cell Division in Live, Intact Tissue. J. Vis. Exp. (159), e60961, doi:10.3791/60961 (2020).

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