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Preparation of Drosophila Larval and Pupal Testes for Analysis of Cell Division in Live, Intact Tissue
JoVE Journal
Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Preparation of Drosophila Larval and Pupal Testes for Analysis of Cell Division in Live, Intact Tissue

Preparation of Drosophila Larval and Pupal Testes for Analysis of Cell Division in Live, Intact Tissue

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08:05 min

May 19, 2020

DOI:

08:05 min
May 19, 2020

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Summary

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The goal of this protocol is to analyze cell division in intact tissue by live and fixed cell microscopy using Drosophila meiotic spermatocytes. The protocol demonstrates how to isolate whole, intact testes from Drosophila larvae and early pupae, and how to process and mount them for microscopy.

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