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Preparation of Drosophila Larval and Pupal Testes for Analysis of Cell Division in Live, Intact Tissue
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Preparation of Drosophila Larval and Pupal Testes for Analysis of Cell Division in Live, Intact Tissue
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May 19, 2020
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The goal of this protocol is to analyze cell division in intact tissue by live and fixed cell microscopy using Drosophila meiotic spermatocytes. The protocol demonstrates how to isolate whole, intact testes from Drosophila larvae and early pupae, and how to process and mount them for microscopy.
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Karabasheva, D., Smyth, J. T. Preparation of Drosophila Larval and Pupal Testes for Analysis of Cell Division in Live, Intact Tissue. J. Vis. Exp. (159), e60961, doi:10.3791/60961 (2020).
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