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Preparation of Drosophila Larval and Pupal Testes for Analysis of Cell Division in Live, Intact Tissue
Bu içeriği görüntülemek için JoVE aboneliği gereklidir. Oturum açın veya ücretsiz deneme sürümünü başlatın.
Preparation of Drosophila Larval and Pupal Testes for Analysis of Cell Division in Live, Intact Tissue
Bölümler
- 00:04Introduction
- 00:46Dissection of Testes from Larvae and Early Pupae
- 03:03Mounting Testes and Live Microscopic Imaging
- 04:47Fixation and Immunostaining
- 05:49Mounting Fixed Testes
- 06:39Results: Optimizing Live Imaging of Cell Division
- 07:26Conclusion
The goal of this protocol is to analyze cell division in intact tissue by live and fixed cell microscopy using Drosophila meiotic spermatocytes. The protocol demonstrates how to isolate whole, intact testes from Drosophila larvae and early pupae, and how to process and mount them for microscopy.
