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Preparation of Drosophila Larval and Pupal Testes for Analysis of Cell Division in Live, Intact Tissue
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発生生物学
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JoVE Journal 発生生物学
Preparation of Drosophila Larval and Pupal Testes for Analysis of Cell Division in Live, Intact Tissue

Preparation of Drosophila Larval and Pupal Testes for Analysis of Cell Division in Live, Intact Tissue

7,718 Views

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08:05 min

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May 19, 2020

DOI:

10.3791/60961-v

DOI:
10.3791/60961-v

•

08:05 min
May 19, 2020

•

8 Views
,
1Department of Anatomy, Physiology, and Genetics, F. Edward Hébert School of Medicine,Uniformed Services University of the Health Sciences

概要

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The goal of this protocol is to analyze cell division in intact tissue by live and fixed cell microscopy using Drosophila meiotic spermatocytes. The protocol demonstrates how to isolate whole, intact testes from Drosophila larvae and early pupae, and how to process and mount them for microscopy.

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Cite this Article

Karabasheva, D., Smyth, J. T. Preparation of Drosophila Larval and Pupal Testes for Analysis of Cell Division in Live, Intact Tissue. J. Vis. Exp. (159), e60961, doi:10.3791/60961 (2020).

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