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Laser Capture Microdissection of Glioma Subregions for Spatial and Molecular Characterization of Intratumoral Heterogeneity, Oncostreams, and Invasion
JoVE Journal
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JoVE Journal Nörobilim
Laser Capture Microdissection of Glioma Subregions for Spatial and Molecular Characterization of Intratumoral Heterogeneity, Oncostreams, and Invasion

Laser Capture Microdissection of Glioma Subregions for Spatial and Molecular Characterization of Intratumoral Heterogeneity, Oncostreams, and Invasion

6,615 Views

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09:09 min

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April 12, 2020

DOI:

10.3791/60939-v

DOI:
10.3791/60939-v

•

09:09 min
April 12, 2020

•

2 Views
, , , , , ,
1Dept. of Neurosurgery,University of Michigan Medical School, 2Dept. of Cell and Developmental Biology,University of Michigan Medical School, 3Dept. of Ophthalmology & Visual Science,University of Michigan Medical School, 4Rogel Cancer Center,University of Michigan Medical School

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Laser microdissection (LMD) is a sensitive and highly reproducible technique that can be used to uncover pathways that mediate glioma heterogeneity and invasion. Here, we describe an optimized protocol to isolate discrete areas from glioma tissue using laser LMD followed by transcriptomic analysis.

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Comba, A., Dunn, P. J., Kish, P. E., Kadiyala, P., Kahana, A., Castro, M. G., Lowenstein, P. R. Laser Capture Microdissection of Glioma Subregions for Spatial and Molecular Characterization of Intratumoral Heterogeneity, Oncostreams, and Invasion. J. Vis. Exp. (158), e60939, doi:10.3791/60939 (2020).

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