Journal
/
神经科学
/
Laser Capture Microdissection of Glioma Subregions for Spatial and Molecular Characterization of Intratumoral Heterogeneity, Oncostreams, and Invasion
JoVE 杂志
神经科学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 神经科学
Laser Capture Microdissection of Glioma Subregions for Spatial and Molecular Characterization of Intratumoral Heterogeneity, Oncostreams, and Invasion

Laser Capture Microdissection of Glioma Subregions for Spatial and Molecular Characterization of Intratumoral Heterogeneity, Oncostreams, and Invasion

DOI:
10.3791/60939-v

•

09:09 min

•

April 12, 2020

•

, , , , , ,
1Dept. of Neurosurgery,University of Michigan Medical School, 2Dept. of Cell and Developmental Biology,University of Michigan Medical School, 3Dept. of Ophthalmology & Visual Science,University of Michigan Medical School, 4Rogel Cancer Center,University of Michigan Medical School

Chapters

  • 00:05Introduction
  • 01:17Animal Perfusion and Brain Preservation
  • 02:01Glioma Brain Tumors Tissue Cryopreservation
  • 02:52Frozen Brain Tumor Tissue Sectioning
  • 03:58Cryopreserved Brain Tissue Section Fixation and Staining
  • 04:48Laser Capture Microdissection
  • 06:30Results: Representative RNA Quality Control and Library Preparation
  • 07:55Conclusion

Summary

自动翻译

自动翻译

Laser microdissection (LMD) is a sensitive and highly reproducible technique that can be used to uncover pathways that mediate glioma heterogeneity and invasion. Here, we describe an optimized protocol to isolate discrete areas from glioma tissue using laser LMD followed by transcriptomic analysis.

Tags

Laser MicrodissectionRNA SequencingIntratumor HeterogeneityMesenchymal CellsTumor InvasionTumor MorphologyMolecular ProfilesTumor ProgressionTherapeutic TargetsBioluminescencePerfusionCryopreservationSpatial CharacterizationMolecular Characterization

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

Laser Capture Microdissection of Drosophila Peripheral Neurons

Non-Laser Capture Microscopy Approach for the Microdissection of Discrete Mouse Brain Regions for Total RNA Isolation and Downstream Next-Generation Sequencing and Gene Expression Profiling

Laser Capture Microdissection of Enriched Populations of Neurons or Single Neurons for Gene Expression Analysis After Traumatic Brain Injury

Laser Capture Microdissection of Neurons from Differentiated Human Neuroprogenitor Cells in Culture

Production of RNA for Transcriptomic Analysis from Mouse Spinal Cord Motor Neuron Cell Bodies by Laser Capture Microdissection

Olfactory Neurons Obtained through Nasal Biopsy Combined with Laser-Capture Microdissection: A Potential Approach to Study Treatment Response in Mental Disorders

Laser Capture Microdissection – A Demonstration of the Isolation of Individual Dopamine Neurons and the Entire Ventral Tegmental Area

Stereotactic Atlas-Guided Laser Capture Microdissection of Brain Regions Affected by Traumatic Injury

Optimization of Laser-Capture Microdissection for the Isolation of Enteric Ganglia from Fresh-Frozen Human Tissue

A Stainless Protocol for High Quality RNA Isolation from Laser Capture Microdissected Purkinje Cells in the Human Post-Mortem Cerebellum

Read Article