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Laser Capture Microdissection of Glioma Subregions for Spatial and Molecular Characterization of Intratumoral Heterogeneity, Oncostreams, and Invasion
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JoVE Journal 神経科学
Laser Capture Microdissection of Glioma Subregions for Spatial and Molecular Characterization of Intratumoral Heterogeneity, Oncostreams, and Invasion

Laser Capture Microdissection of Glioma Subregions for Spatial and Molecular Characterization of Intratumoral Heterogeneity, Oncostreams, and Invasion

6,616 Views

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09:09 min

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April 12, 2020

DOI:

10.3791/60939-v

DOI:
10.3791/60939-v

•

09:09 min
April 12, 2020

•

2 Views
, , , , , ,
1Dept. of Neurosurgery,University of Michigan Medical School, 2Dept. of Cell and Developmental Biology,University of Michigan Medical School, 3Dept. of Ophthalmology & Visual Science,University of Michigan Medical School, 4Rogel Cancer Center,University of Michigan Medical School

概要

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Laser microdissection (LMD) is a sensitive and highly reproducible technique that can be used to uncover pathways that mediate glioma heterogeneity and invasion. Here, we describe an optimized protocol to isolate discrete areas from glioma tissue using laser LMD followed by transcriptomic analysis.

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Cite this Article

Comba, A., Dunn, P. J., Kish, P. E., Kadiyala, P., Kahana, A., Castro, M. G., Lowenstein, P. R. Laser Capture Microdissection of Glioma Subregions for Spatial and Molecular Characterization of Intratumoral Heterogeneity, Oncostreams, and Invasion. J. Vis. Exp. (158), e60939, doi:10.3791/60939 (2020).

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