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Isolation and Characterization of Extracellular Vesicles Produced by Iron-limited Mycobacteria
JoVE Journal
İmmünoloji ve Enfeksiyon
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JoVE Journal İmmünoloji ve Enfeksiyon
Isolation and Characterization of Extracellular Vesicles Produced by Iron-limited Mycobacteria

Isolation and Characterization of Extracellular Vesicles Produced by Iron-limited Mycobacteria

6,947 Views

08:27 min

October 31, 2019

DOI:

08:27 min
October 31, 2019

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Özet

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Mycobacterium tuberculosis shows increased production and release of extracellular vesicles in response to low iron conditions. This work details a protocol for generating low iron conditions and methods for the purification and characterization of mycobacterial extracellular vesicles released in response to iron deficiency.

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