/
/
Isolation and Characterization of Extracellular Vesicles Produced by Iron-limited Mycobacteria
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
Isolation and Characterization of Extracellular Vesicles Produced by Iron-limited Mycobacteria
6,947 Views
•
08:27 min
•
October 31, 2019
DOI:
Summary
Automatically generated
Mycobacterium tuberculosis shows increased production and release of extracellular vesicles in response to low iron conditions. This work details a protocol for generating low iron conditions and methods for the purification and characterization of mycobacterial extracellular vesicles released in response to iron deficiency.
Read Article
Cite this Article
Gupta, S., Marcela Rodriguez, G. Isolation and Characterization of Extracellular Vesicles Produced by Iron-limited Mycobacteria. J. Vis. Exp. (152), e60359, doi:10.3791/60359 (2019).
Copy