/
/
Isolation and Characterization of Extracellular Vesicles Produced by Iron-limited Mycobacteria
É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Isolation and Characterization of Extracellular Vesicles Produced by Iron-limited Mycobacteria
6,963 Views
•
08:27 min
•
October 31, 2019
DOI:
Summary
Automatically generated
Mycobacterium tuberculosis shows increased production and release of extracellular vesicles in response to low iron conditions. This work details a protocol for generating low iron conditions and methods for the purification and characterization of mycobacterial extracellular vesicles released in response to iron deficiency.
Read Article
Cite this Article
Gupta, S., Marcela Rodriguez, G. Isolation and Characterization of Extracellular Vesicles Produced by Iron-limited Mycobacteria. J. Vis. Exp. (152), e60359, doi:10.3791/60359 (2019).
Copy