Dergi
/
İmmünoloji ve Enfeksiyon
/
Targeting Drugs to Larval Zebrafish Macrophages by Injecting Drug-Loaded Liposomes
JoVE Journal
İmmünoloji ve Enfeksiyon
Bu içeriği görüntülemek için JoVE aboneliği gereklidir.  Oturum açın veya ücretsiz deneme sürümünü başlatın.
JoVE Journal İmmünoloji ve Enfeksiyon
Targeting Drugs to Larval Zebrafish Macrophages by Injecting Drug-Loaded Liposomes

Targeting Drugs to Larval Zebrafish Macrophages by Injecting Drug-Loaded Liposomes

DOI:
10.3791/60198-v

•

11:31 min

•

February 18, 2020

•

, , , , ,
1Department of Molecular Medicine and Pathology, Faculty of Medical and Health Sciences,University of Auckland, 2School of Pharmacy, Faculty of Medical and Health Sciences,University of Auckland, 3School of Medicine, Faculty of Medical and Health Sciences,University of Auckland

Bölümler

  • 00:05Introduction
  • 01:20Drug-loaded Marina Blue-Labeled Lipsome Preparation
  • 03:24Liposome Size and Zeta Potential Characterization and Entrapment Efficiency and Drug Loading Calculation
  • 04:11Preparation and Liposome Injection
  • 06:47Confocal Imaging and Image Analysis
  • 09:03Results: Representative Effects of Drug-loaded Lipsome Injection
  • 10:48Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

Here, we describe the synthesis of drug-loaded liposomes and their microinjection into larval zebrafish for the purpose of targeting drug delivery to macrophage-lineage cells.

Etiketler

LiposomesMacrophagesZebrafishDrug DeliveryMitochondrial Reactive Oxygen SpeciesPoloxamer 188ExtrusionUltracentrifugationLive Cell Imaging

Article

Embed

ÇALMA LİSTESİNE EKLE

Usage Statistics

İlgili Videolar

Humanized Mouse Model to Study Bacterial Infections Targeting the Microvasculature

Use of Shigella flexneri to Study Autophagy-Cytoskeleton Interactions

Assessing Anti-fungal Activity of Isolated Alveolar Macrophages by Confocal Microscopy

Highly Efficient Transfection of Human THP-1 Macrophages by Nucleofection

Modeling Mucosal Candidiasis in Larval Zebrafish by Swimbladder Injection

Proteomic Profiling of Macrophages by 2D Electrophoresis

Non-invasive Imaging of the Innate Immune Response in a Zebrafish Larval Model of Streptococcus iniae Infection

Quantification of Cytosolic vs. Vacuolar Salmonella in Primary Macrophages by Differential Permeabilization

Phenotypic Characterization of Macrophages from Rat Kidney by Flow Cytometry

Using Zebrafish Models of Human Influenza A Virus Infections to Screen Antiviral Drugs and Characterize Host Immune Cell Responses

Read Article