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Targeting Drugs to Larval Zebrafish Macrophages by Injecting Drug-Loaded Liposomes
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Targeting Drugs to Larval Zebrafish Macrophages by Injecting Drug-Loaded Liposomes

Targeting Drugs to Larval Zebrafish Macrophages by Injecting Drug-Loaded Liposomes

DOI:
10.3791/60198-v

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11:31 min

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February 18, 2020

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, , , , ,
1Department of Molecular Medicine and Pathology, Faculty of Medical and Health Sciences,University of Auckland, 2School of Pharmacy, Faculty of Medical and Health Sciences,University of Auckland, 3School of Medicine, Faculty of Medical and Health Sciences,University of Auckland

Chapters

  • 00:05Introduction
  • 01:20Drug-loaded Marina Blue-Labeled Lipsome Preparation
  • 03:24Liposome Size and Zeta Potential Characterization and Entrapment Efficiency and Drug Loading Calculation
  • 04:11Preparation and Liposome Injection
  • 06:47Confocal Imaging and Image Analysis
  • 09:03Results: Representative Effects of Drug-loaded Lipsome Injection
  • 10:48Conclusion

Summary

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Here, we describe the synthesis of drug-loaded liposomes and their microinjection into larval zebrafish for the purpose of targeting drug delivery to macrophage-lineage cells.

Tags

LiposomesMacrophagesZebrafishDrug DeliveryMitochondrial Reactive Oxygen SpeciesPoloxamer 188ExtrusionUltracentrifugationLive Cell Imaging

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