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Targeting Drugs to Larval Zebrafish Macrophages by Injecting Drug-Loaded Liposomes
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Immunologie und Infektion
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JoVE Journal Immunologie und Infektion
Targeting Drugs to Larval Zebrafish Macrophages by Injecting Drug-Loaded Liposomes

Targeting Drugs to Larval Zebrafish Macrophages by Injecting Drug-Loaded Liposomes

7,478 Views

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11:31 min

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February 18, 2020

DOI:

10.3791/60198-v

DOI:
10.3791/60198-v

•

11:31 min
February 18, 2020

•

7 Views
, , , , ,
1Department of Molecular Medicine and Pathology, Faculty of Medical and Health Sciences,University of Auckland, 2School of Pharmacy, Faculty of Medical and Health Sciences,University of Auckland, 3School of Medicine, Faculty of Medical and Health Sciences,University of Auckland

Summary

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Here, we describe the synthesis of drug-loaded liposomes and their microinjection into larval zebrafish for the purpose of targeting drug delivery to macrophage-lineage cells.

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LiposomesMacrophagesZebrafishDrug DeliveryMitochondrial Reactive Oxygen SpeciesPoloxamer 188ExtrusionUltracentrifugationLive Cell Imaging

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Cite this Article

Linnerz, T., Kanamala, M., Astin, J. W., Dalbeth, N., Wu, Z., Hall, C. J. Targeting Drugs to Larval Zebrafish Macrophages by Injecting Drug-Loaded Liposomes. J. Vis. Exp. (156), e60198, doi:10.3791/60198 (2020).

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