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来自胚胎GFP-表达小鼠的Ex Vivo Oculo运动切片培养,用于对Oculo运动神经外生长进行时移成像
JoVE Journal
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JoVE Journal Nörobilim
Ex Vivo Oculomotor Slice Culture from Embryonic GFP-Expressing Mice for Time-Lapse Imaging of Oculomotor Nerve Outgrowth
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来自胚胎GFP-表达小鼠的Ex Vivo Oculo运动切片培养,用于对Oculo运动神经外生长进行时移成像

DOI:
10.3791/59911-v

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06:04 min

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July 16, 2019

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, , ,
1Department of Ophthalmology,Boston Children’s Hospital, 2Department of Ophthalmology,Harvard Tıp Fakültesi, 3F.M. Kirby Neurobiology Center,Boston Children’s Hospital, 4Department of Neurology,Boston Children’s Hospital, 5Department of Neurology,Harvard Tıp Fakültesi, 6Howard Hughes Medical Institute

Bölümler

  • 00:04Başlık
  • 00:51Pregnancy Confirmation and E10.5 Embryo Harvest and Slice Preparation
  • 04:15Results: Representative Ex Vivo Oculomotor Slice Culture Development and Pitfalls
  • 05:30Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

前体切片测定允许实时成像奥库运动神经外生。切片通过嵌入E10.5 Isl MN:GFP胚胎在生长中,在振动体上切片,并在一个顶的培养箱中生长。斧子引导通路的作用是通过向培养培养物添加抑制剂来评估的。

Etiketler

Ex VivoOculomotorSlice CultureEmbryonicGFP-expressingTime-lapse ImagingOculomotor Nerve OutgrowthAxon GuidanceOcular Motor SystemVibratomeAgaroseSlice Buffer

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