/
/
Ex Vivo Oculomotor Slice Culture from Embryonic GFP-Expressing Mice for Time-Lapse Imaging of Oculomotor Nerve Outgrowth
Se requiere una suscripción a JoVE para ver este contenido. Inicie sesión o comience su prueba gratuita.
Ex Vivo Oculomotor Slice Culture from Embryonic GFP-Expressing Mice for Time-Lapse Imaging of Oculomotor Nerve Outgrowth
8,102 Views
•
06:04 min
•
July 16, 2019
DOI:
1Department of Ophthalmology,Boston Children’s Hospital, 2Department of Ophthalmology,Harvard Medical School, 3F.M. Kirby Neurobiology Center,Boston Children’s Hospital, 4Department of Neurology,Boston Children’s Hospital, 5Department of Neurology,Harvard Medical School, 6Howard Hughes Medical Institute
Summary
Automatically generated
An ex vivo slice assay allows oculomotor nerve outgrowth to be imaged in real time. Slices are generated by embedding E10.5 IslMN:GFP embryos in agarose, slicing on a vibratome, and growing in a stage-top incubator. The role of axon guidance pathways is assessed by adding inhibitors to the culture media.
Read Article
Cite this Article
Whitman, M. C., Bell, J. L., Nguyen, E. H., Engle, E. C. Ex Vivo Oculomotor Slice Culture from Embryonic GFP-Expressing Mice for Time-Lapse Imaging of Oculomotor Nerve Outgrowth. J. Vis. Exp. (149), e59911, doi:10.3791/59911 (2019).
Copy